Analysis Note

ControlA431 cell lysate

Application

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected NLRP10 in Flag-tagged NRLP10 transfected HeLa cells (Lautz, K., et al. (2012). Cell Microbiol. 14(10:1568-1583.).

Western Blotting Analysis: A representative lot from an independent laboratory detected NLRP10 primary epidermal fibroblasts transfected with a non-targeting control siRNA, and demonstrated a loss of signal in primary epidermal fibroblasts transfected with NLRP10-specific RNA (Lautz, K., et al. (2012). Cell Microbiol. 14(10:1568-1583.).

Flow Cytometry Analysis: 4 µg from a representative lot detected NLRP10 in 1X10E6 HeLa and Jurkat cells.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Anti-NLRP10 Antibody, clone 8H2 is a highly specific rat monoclonal antibody, that targets NLRP10 & has been tested in western blotting, Flow Cytometry & ICC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NACHT, LRR and PYD domains-containing protein 10 (NLRP10) is also called Nucleotide-binding oligomerization domain protein 8. NLRP10 has anti-inflammatory activity and plays a role in immune response against infections from bacteria including C.albicans by contributing to proinflammatory cytokine release. NLRP10 is widely expressed with the highest levels found in heart, brain and skeletal muscle, as well as liver, colon, dermis and epidermis.

Immunogen

Recombinant protein corresponding to human NLRP10.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1 mg/mL of this antibody detected NLRP10 in 10 µg of A431 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~72 kDa observed